Category Archives: Techniques

xenopat

Xenopat: Your Tumor in a Mouse

Labcritics visited Xenopat, an innovative company dedicated to the orthotopic implantation of human tumors in mice. This technology allows Xenopat to offer services to companies developing new anticancer drugs and personalized cancer treatments. We had the pleasure of speaking with Dr. Anna Portela, CEO and co-founder, and Dr. Alberto Villanueva, CSO and co-founder. For more […]

iproteos

Teresa Tarragó: “Crowdfunding is a Source of Financing for Early Stage Biotechs”

Labcritics was at Iproteos’ facilities talking to Teresa Tarragó, CEO and co-founder of this innovative company. Iproteos combines computational chemistry with modern synthesis methods to generate peptidomimetics with pharmacological activity against diseases such as schizophrenia. We had the opportunity to know about the possibilities of crowdfunding for biotech projects and the technology used in their laboratory. […]

crispr-cas9

CRISPR as a Recorder of Molecular Activity

Researchers from Harvard University have succesfully proved that CRISPR can work as a recorder of molecular events. Synthetic DNA molecules introduced in bacteria over a defined timespan were integrated by the CRISPR/Cas system in DNA arrays in the exact same order of introduction. The results, published in the journal Science, pave the way for using CRISPR to […]

crispr-cas9

CRISPR/Cpf1 Edits Mice Embryos Without Off-Target Effects

Researchers at the IBS Center for Genome Editing in South Korea have successfully created mutant mice using CRISPR/Cpf1. The alternative to the well-known CRISPR/Cas9 system can edit genomes with exquisite precision and is highly programmable. The findings -published in two papers that appeared this month in the journal Nature Biotechnology-, together with previously known advantages of Cpf1 over […]

Ultrashort Laser Pulses Reduce Off-Target Effects of Radiotherapy

Researchers from the Tata Institute of Fundamental Research in India have developed an innovative technique to cause DNA damage in a controlled manner. Short, intense pulses of laser light can be directed to a therapeutic target without damaging the surrounding area. The findings were published in the journal Scientific Reports. Radiotherapy is a treatment that uses […]

Size Exclusion Chromatography Tips and Tricks

Size exclusion chromatography (SEC) is a technique that allows to perform molecular separation by size. Some common SEC applications are protein purification, protein-protein interaction assays, the analysis of protein aggregation processes and protein stability studies. Inger Salomonsson from GE Healthcare presented at Analytica very useful tips and tricks to improve SEC performance. First, the system configuration must […]

Quantification of Genome Editing Events Using ddPCR

Bio-Rad presented at Analytica the ultra-sensitive properties of digital droplet PCR (ddPCR) to quantify homology directed repair (HDR) and non-homologous end joining (NHEJ) events in genome editing experiments. Adam Bemis talked about the powerful applications of Bio-Rad’s QX200 Droplet Digital PCR in cutting-edge research and next-gen medicine. ddPCR divides the sample in thousands of droplets, […]

Droplet Digital PCR to Measure Tumor Load

Christof Winter from Technische Universität München presented at Analytica the innovative use of droplet digital PCR to measure tumor load by non-invasive means. The technique takes advantage of the cell-free DNA circulating in blood, which is found in higher concentration in cancer patients. Cell-free DNA (cfDNA) is found in blood plasma at concentrations of 10–100 […]