Category Archives: Genome Editing

CRISPR Kit

Humans Found to Hold Immunity to CRISPR-Cas9

CRISPR-Cas9, the 21st Century’s most promising genetic modification tool has shown to be unusable in humans, thanks to adaptive immunity already present in human beings. In a pre-print paper published by researchers at Stanford University, more than 65 percent of human subjects had antibodies that would fight Cas9 proteins propagation in human beings. More specifically 79% […]

Software for Scientists

Benchling Adds Protein Tools to its Genetic Toolset

Benchling is adding new protein tools to its version control collaboration genetic toolkit. San Francisco based online gene-editing and collaboration platform, Benchling has introduced new set of tools helping molecular biologists to identify and annotate protein sequences. It enables directly importing proteins from a DNA sequence by highlighting a section of DNA that the researcher is currently working […]

crispr-cas9

CRISPR as a Recorder of Molecular Activity

Researchers from Harvard University have succesfully proved that CRISPR can work as a recorder of molecular events. Synthetic DNA molecules introduced in bacteria over a defined timespan were integrated by the CRISPR/Cas system in DNA arrays in the exact same order of introduction. The results, published in the journal Science, pave the way for using CRISPR to […]

phage

Anti-CRISPR Proteins from Prophages Discovered in Proteobacterial Genomes

Researchers from the University of Toronto and the University of Otago have discovered five new proteins that block the bacterial CRISPR/Cas immune system. The anti-CRISPR proteins are encoded in genes belonging to bacteriophage sequences inserted in the bacterial genome. The new findings, published in the journal Nature Microbiology, enlarge the list of known anti-CRISPR systems. CRISPR/Cas is a bacterial immune […]

crispr-cas9

CRISPR/Cpf1 Edits Mice Embryos Without Off-Target Effects

Researchers at the IBS Center for Genome Editing in South Korea have successfully created mutant mice using CRISPR/Cpf1. The alternative to the well-known CRISPR/Cas9 system can edit genomes with exquisite precision and is highly programmable. The findings -published in two papers that appeared this month in the journal Nature Biotechnology-, together with previously known advantages of Cpf1 over […]

Quantification of Genome Editing Events Using ddPCR

Bio-Rad presented at Analytica the ultra-sensitive properties of digital droplet PCR (ddPCR) to quantify homology directed repair (HDR) and non-homologous end joining (NHEJ) events in genome editing experiments. Adam Bemis talked about the powerful applications of Bio-Rad’s QX200 Droplet Digital PCR in cutting-edge research and next-gen medicine. ddPCR divides the sample in thousands of droplets, […]

crispr-cas9

New CRISPR Nuclease Cuts Both DNA and RNA

CRISPR/Cas9 co-discoverer Emmanuelle Charpentier and collaborators from Germany and Sweden have found that Cas nuclease Cpf1 cleaves DNA and RNA. Cpf1 cuts a precursor of crRNA to produce the mature form; it requires only one RNA strand to cleave the target DNA and is smaller that Cas9. The findings were published in the journal Nature. CRISPR/Cas systems have been […]

Veritas Genomics

It’s Here!! $1,000 Whole Genome Sequencing, With Results Browsable on Smartphones

Veritas Genetics has announced its $1,000 whole genome sequencing service whose results will be browsable on smartphones. The MyGenome service announced last year and set to be officially launching this month will give its clients and their physicians deep insights into their whole genome. Veritas Genomics is going one step ahead and making the genomic database […]