EMD Millipore, the life sciences division of Merck KGaA has introduced the new CpGenome Direct Prep Bisulfite Modification kit. This new kit allows bisulfite conversion of DNA from blood, cells, tissues and FFPE samples without any need for purification.
The new CpGenome Direct Prep Bisulfite Modification Kit can be used to conduct bisulfite conversion to determine methylation pattern in DNA with more than 99.5% conversion efficiencies, while using samples as less as 10 cells or 50 pg of DNA. This new kit streamlines the bisulfite analysis workflow by providing detailed guidance to researchers working with limited quantities of samples and it is also ideal for downstream analysis involving methylation specific PCR, restriction digestion sequencing and microarray hybridization methods. And it also does not require additional precipitation steps for DNA recovery as its in-column desulfonation eliminates the need for extra steps and produces consistent results.