HPLC Comaprision

Specifications
Agilent 1220 Infinity Mobile LC Solution
Agilent 1260 Infinity Quaternary LC System
Agilent 1260 Infinity Binary LC System
Key benefits• Dual gradient pump with integrated degasser, 600 bar, up to 10 mL/ min,
for reliable and robust analysis
• Vial sampler with injector programming, expandable to walk-up
• 80 °C oven for one 25-cm column
• Variable wavelength or diode array detector with 80 Hz sampling rate for
highest sensitivity
• Mobile Upgrade Kit with attenuation unit for vibration resistance
• Walk-Up Upgrade Kit with external tray for easy sharing of one instrument
for LC/MS analysis
Pump
Hydraulic systemDual plunger in series pump with proprietary servo-controlled variable stroke drive, fl oating plungers and passive inlet valve Dual piston in series pump with proprietary servo-controlled variable stroke drive, floating
pistons
Dual piston in series pump with servo-controlled variable stroke drive, floating pistons
Settable fl ow range 0.001 – 10 mL/min, in 0.001 mL/ min increments 0.001 – 10 mL/min, in 0.001 mL/min incrementsSet points 0.001 - 10 mL/min, in 0.001 mL/min increments
Flow range0.2 – 10 mL/min0.2 – 10.0 mL/min0.2 - 10.0 mL/min
Flow precision<0.07 % RSD, or < 0.02 min SD whatever is greater, based on retention time at constant room temperature< 0.07 % RSD, or < 0.02 min SD whatever is greater, based on retention time at constant room
temperature
<= 0.07 % RSD or <= 0.02 min SD, whatever is greater; based on retention time at constant room temperature
Flow accuracy± 1 % or 10 ?l/min whatever is greater± 1 % or 10 µL/min whatever is greater, pumping degassed H2O at 10 Mpa± 1 % or 10 µL/min whatever is greater; pumping degassed H2O at 10 Mpa (100 bar)
Pressure Operating range0 – 60 MPa (0 – 600 bar, 0 – 8700 psi) up to 5 mL/min Operating range 0 – 20 MPa (0 – 200 bar, 0 – 2950 psi) up to 10 mL/min (all versions)Operating range 0 – 60 MPa (0 – 600 bar, 0 – 8700 psi) up to 5 mL/min
Operating range 0 – 20 MPa (0 – 200 bar, 0 – 2950 psi) up to 10 mL/min
1260 Infinity Quaternary pump: Operating range up to 60 MPa (600 bar, 8700 psi) up to 5 mL/min | Operating range up to 20 MPa (200 bar, 2950 psi) up to 10 mL/min 1260 Infinity Quaternary pump VL: Operating range up to 40 MPa (400 bar, 5880 psi) up to 5 mL/min | Operating range up to 20 MPa (200 bar, 2950 psi) up to 10 mL/min
Pressure pulsation < 2 % amplitude (typically < 1 %), at 1 mL/min isopropanol, at all pressures > 1 MPa (10 bar)< 2 % amplitude (typically < 1.3 %), or < 3 bar at 1 mL/min isopropanol, at all pressures > 10 bar
(147 psi)
< 2 % amplitude (typically < 1.3 %) or < 0.3 MPa (3 bar), whatever is greater, at 1 mL/min isopropanol, at all pressures > 1 MPa (10 bar, 147 psi)
Recommended pH range1.0 – 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel1.0 – 12.5 , solvents with pH < 2.3 should not contain acids which attack stainless steel1.0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
Gradient formation (gradient pump or optional; two solvents)Low pressure dual mixing/gradient capability
using proprietary high-speed proportioning valve
Delay volume 600 – 900 µL,
dependent on back pressure
Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning
valve
Low pressure quaternary mixing/gradient capability using high-speed proportioning valve
Composition range0 – 95 % or 5 – 100 %, user selectable 0 – 95 % or 5 – 100 %, user selectable0 - 95 % or 5 - 100 %, user selectable
Composition precision< 0.2 % RSD or < 0.04 min SD, whichever is greater,
1 mL/min; based on retention time at constant
room temperature
< 0.2 % RSD, or < 0.04 min SD whatever is greater, at 0.2 and 1 mL/min< 0.2 % RSD or < 0.04 min SD, whatever is greater, at 1 mL/min; based on retention time at constant room temperature
Valid Metering HeadsValid with standard 100 µL metering head installed Valid with standard 900 µL metering head installed
Pressure Operating range 0 – 60 MPa (0 – 600 bar, 0 – 8700 psi)0 - 60 MPa (0 - 600 bar, 0 - 8850 psi)100 µL: Up to 600 bar (60 MPa or 8702 psi) | 900 µL: Up to 200 bar (20 MPa or 2901 psi)
Injection range0.1 – 100 ?L in 0.1 ?L increments
Up to 1500 ?L with multiple draw
(hardware modifi cation required)
0.1 - 100 µL in 0.1 µL increments (recommended 1 µL increments)
Up to 1500 µL with multiple draw (hardware modification required)
100 µL: 0.1- 100 µL in 0.1 µL increments
Up to 1500 µL with multiple draw (hardware modification required)
900 µL: 0.1 - 900 µL in 0.1 µL increments (recommended 1 µL increments)
Up to 1800 µL with multiple draw (hardware modification required)
Replicate injections1 – 99 from one vial1 – 99 from one vial
Precision< 0.25 % RSD from 5 – 100 ?L, < 1% RSD 1 – 5 ?L variable volumeTypically < 0.25 % RSD of peak areas from 5 - 100 µL, Typically < 1 % RSD of peak areas from
1 - 5 µL,
100 µL: <0.25% RSD from 5-100 µL, <1% from 1-5 µL, variable volume
900 µL: Typically < 0.5 % RSD of peak areas from 5 - 2000 µL
Typically < 1 % RSD of peak areas from 2000 - 5000 µL
Typically < 3 % RSD of peak areas from 1 - 5 µL
Variable volume
Minimum sample volume1 ?L from 5 ?L sample in 100 ?L microvial, or 1 ?L
from 10 ?L sample in 300 ?L microvial
1 µL from 5 µL sample in 100 µL microvial, or 1 µL from 10 µL sample in 300 µL microvial
CarryoverTypically < 0.1 %, < 0.05 % with external needle cleaning Typically < 0.1 %, < 0.05 % with external needle cleaning100 µL: Typically < 0.1 %, < 0.05 % with external needle cleaning
900 µL: Typically < 0.1 %, < 0.05 % with external needle cleaning
Sample viscosity range0.2 – 50 cp0.2 – 50 cp100 µL: 0.2 - 5 cp
900 µL: 0.2 - 5 cp
Sample capacity100 × 2-mL vials in 1 tray
40 × 2-mL vials in ½ tray
15 × 6-mL vials in ½ tray (Agilent vials only)
100 × 2 mL vials in 1 tray
40 × 2 mL vials in ½ tray
15 × 6 mL vials in ½ tray (Agilent vials only)
100 x 2-mL vials in 1 tray
40 x 2-mL vials in 1/2 tray
15 x 6-mL vials in 1/2 tray (Agilent vials only)
Injection cycle timeTypically 50 s depending on draw speed and
injection volume
50 s for draw speed 200 µL/min, ejection speed 200 µL/min, injection volume 5 µLTypically 50 s depending on draw speed and injection volume
Column Oven Temperature range5 °C above ambient to 80 °C10 degrees below ambient to 80 °C
up to 80 °C: flow rates up to 5 mL/min
10 degrees below ambient to 80 °C
up to 80 °C: flow rates up to 5 mL/min
Column Oven Temperature stability± 0.15 °C± 0.15 °C± 0.15 °C
Column Oven Temperature accuracy± 0.8 °C with calibration ± 0.5 °C± 0.8 °C
± 0.5 °C With calibration
± 0.8 °C
± 0.5 °C With calibration
Column Oven Column capacityOne 25-cm columnThree 30 cmThree 30 cm
Detector typeVariable Wavelength Detector (G4286B, G4288B, G4290B)Variable Wavelength Detector (VWD)(G1314F)Variable Wavelength Detector (G1314F)
Light sourceDeuterium lampDeuterium lampDeuterium lamp
Wavelength range190 – 600 nm190 – 600 nm
Short term noise± 0.35 × 10-5 AU, at 230 nm± 0.25·10-5 AU at 230 nm < ± 0.25 x 10 -5 AU, at 230 nm*
Drift3 × 10-4 AU/hr, at 254 nm< 1·10-4 AU/h at 230 nm1 x 10-4AU/hr, at 230 nm
Linearity> 2 AU (5 %) upper limit > 2.5 AU (5 %) at 265 nm>2.5 AU upper limit
Wavelength accuracy± 1 nm; self-calibration with deuterium lines,
verifi cation with holmium oxide fi lter
± 1 nm± 1 nm, self-calibration with deuterium lines, verification with holmium oxide filter
Band width6.5 nm typical6.5 nm typical6.5 nm typical over whole wavelength range
Diode Array DetectorG4294B onlyG4212BG4212B
Detector type1024-element diode array1024-element photodiode array1024-element diode array
Light sourceDeuterium and tungstenDeuterium lampDeuterium
Number of signals88
Maximum sampling rate80 Hz80 Hz80 Hz (both spectra and signals)
Short-term noise< ± 0.7 x 10-5 AU at 254/4 nm and at 750 nm, TC 2 s< ± 3 × 10-6 AU at 230 nm/4 nm< ± 3 x 10-6 AU at 230/4 nm, slit width 4 nm,
TC 2 sec, ASTM with 10 mm Max-Light cartridge cell

Typically <± 0.6 x 10-6 AU/cm at 230/4 nm, slit width 4 nm,
TC 2 sec, ASTM with 60 mm Max-Light cartridge cell
Drift< 0.9 x 10-3 AU/hr at 254 nm< 0.5 × 10-3 AU/hr at 230 nm> 2.0 AU (5 %) at 265 nm
Typically 2.5 AU (5 %)
Linearity> 2.0 AU (5 %) at 265 nm> 2.0 AU (5 %) at 265 nm
Wavelength range190-950 nm190 – 640 nm190-640 nm
Wavelength accuracy± 1 nm, self-calibration with deuterium lines
verifi cation with holmium oxide fi lter
± 1 nm± 1 nm, self-calibration with deuterium lines
Slit widthProgrammable: 1, 2, 4, 8, 16 nm4 nm4 nm (fixed)
Diode width< 1 nm~ 0.5 nm~ 0.5 nm