New NGS Platforms for Targeted Sequencing



Thermo Fisher presents two new next-generation sequencing (NGS) systems that simplify targeted sequencing. The Ion S5 and Ion S5 XL platforms are benchtop systems based on the Torrent technology that can be used in research areas like translational cancer research, microbial identification, inherited disorders or infectious agents.

The key aspects of the Ion S5 and Ion S5 XL systems are their simplicity and streamlined workflow. Both systems can sequence gene panels, small genomes, exomes and transcriptomes, and custom assays are also possible. Reagents are contained in ‘plug-and-play´ cartridges, reducing manipulation time. It takes 15 minutes to set up a sequencing run, and just 45 minutes of work from DNA to data. This is possible thanks to the Ion Chef System, which combined with Ion AmpliSeq automatizes library construction, template preparation and chip loading.

Targeted sequencing is now accessible to academic, translational and clinical research labs. The Ion S5 system is more appropriate to academic or translational labs that need an all-in-one sequencer without turnaround time constraints. The Ion S5 XL system is more suited to clinical research labs that every day need a fast turnaround or a high sample throughput. The platforms include 3 new Ion chips (Ion 520, 530 and 540) with outputs of 5M to 80M sequencing reads in 2.5 hours.

The Ion Torrent technology has been successfully used in the Saudi Human Genome Program, a research project that has produced important papers about inherited diseases. Moreover, translational researchers have tested the platform and found that the Ion S5 XL system reduces the run time in 2 hours (from 4.5 hours to 2.5 hours), and cuts the data analysis time to half.

Ion S5 Features
  • Simplicity: less that 15 minutes of sequencer hands-on time; less than 45 minutes hands-on time from DNA to data.
  • Speed: from DNA to data in 24 hours; 2.5 to 4 hours per sequencing run.
  • Small sample input: 10 ng of DNA or RNA are enough for mutational or gene expression profiles.
  • Scalability: the same sequencer can be used for cancer, inherited disease, infectious disease or reproductive genomics research.

Source: selectscience