The genetic modification of mammalian cell lines just became easier with the introduction of new CRISPR/Cas9 full gene editing tool by AMSBIO. This tool can be used for modifying any mammalian cell by targeting any gene without using foreign genetic material.
The new AMSBIO CRISPR/Cas9 gene editing tool carries out the process by utilizing the Cas9 protein, co-expressed with a guide RNA vector from human U6 polymerase III promoter. Editing the genome using CRISPR/Cas9 tool involves inserting a functional cassette, synthesized in a rescue donor vector into the unwound DNA cleaved by Cas9 upon recognition of the target sequence by guide RNA.
This new tool is simpler, more powerful and efficient that the existing gene editing tools like TALENs and zinc finger. CRISPR/Cas9 is ideal for bi-allelic gene modification operations, creating knock-in, knock-out and mutations of any gene in any mammalian cell line.